Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Determine concentration using the Beer-Lambert LawPage 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Molecular Devices' microplate readers. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. VMax . • Photometric range: 0. The soil was amended with either 1. BioTek Instruments Synergy Neo2 Multi-Mode Reader Fluorescence Measuring Equipment. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). Microplate Readers include the M3, M4, M5, and M5e models. Unparalleled performance on a personalized platform. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. $542. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. x. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. 020 fmol 0. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Tristar 3 Multimode Microplate Reader. • 0. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. 4 (Molecular Devices cat. Dual injectors for kinetic assays. FlexIII. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. #C1500) Control standard endotoxin (ACC cat. x. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Next-generation luminescence plate reader. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Selects either 96-well or 384-well plate for a stand-alone read. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Our microplate. Top reading gives greater sensitivity for 96 well plates, unless you have cells adhering to the bottom of the well. Page 1 of 8 . of Dispensers:Molecular Devices deliver high throughput while showing good correlation with manual. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. For a 96-well plate it takes a total of 27 seconds to switch between wells and then you have to add the actual injection time based on the injection rate entered by the user. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. 96- & 384-well automated washer, multiwash™+ automated washer, 96- and 384-well plates, washer maintenance kit. Automated live cell imaging – cell counting and confluence. Fluorescence Microplate Reader Comparison Chart. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Products : Ionworks HT, Ionworks Quattro. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible. Related products. The Absorbance 96 Plate Reader is designed to run everyday assays with ease and speed. (Molecular Devices cat. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). In the latter case, 8 wells in each column are perfused in series and the 12 columns are. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. The CellTiter-Glo assay was used to measure ATP. 96-well) while others have flexibility to. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. Plate Shaking. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Absorbance equation. (96- and 384-well with pipetting) 6-384 (96- and 384-well). Users can choose to process all plates within the magazine or select a specific number. No accessories are needed and SoftMax® Pro Software. Supersedes 1. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Selects either 96-well or 384-well plate for a stand-alone read. x. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. p-Nitroaniline - Catalog No. Microplate washers are designed for effortless use with 96- or 384-well plates in routine ELISA applications. Other. The IC50s by cell rounding and LysoTracker. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. We would like to show you a description here but the site won’t allow us. (one set per assay) to clear 96-well microplates. 96/384-well plate formats. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 5in (46. Robust shaking and consistent temperature control optimize bacteria and yeast cell growth. Sample by eye within . Figure 1-2 Match chamfered corners of slide and adapter 2. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettorElisa Plate Readers and Microplate Readers at Biocompare. 000 to 2. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. 5 ng per well. Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. flat-bottom: 50-60 µl. Component A (2 vials*)Figure 1. Loading Buffer was also added to a set of cell-free wells for background controls. The procedures in this protocol support the 96-well reaction plate. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Reaction formats The MagMAX™-96 DNA Multi-Sample Kit can accommodate a broad range of throughputs. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Compare this item. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. This approach enables uniform light dose application to 9 wells (a 3-by-3 matrix in a 96-well plate) simultaneously, which can be leveraged to shorten the duration of PDT experiments (Fig. 1. Cell line development teams will realize savings on time and resources, as well as faster time-to-market by utilizing a single technology for measuring a critical quality attribute duringOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. 5°C at 37°C. Fluorescence Microplate Reader Comparison Chart. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Get Quote . With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. x. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Categories: Plate Readers. 00. 00 USD. Wells of 96-well plates (NEST) were pre-coated with 100 μg/ml of collagen solution for 1 hour. Product Compare 0. Turn on Plate Reader and wait for it to complete its start-up routine. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. No. 9000-0756. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. In SoftMax Pro, Dual Read mode was selected. , Corning, NY) or Greiner. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. Quartz Plate found in: Thick Quartz Ground and Polished Plates, Solid Quartz 96 Well Clear Plate with Clear Base, Quartz Parallel Plate Probes for DMA 7. Contact our support team with your questions regarding Molecular Devices products and applications. Cell viability and cytoxicity assays. Sort By: Show:. We would like to show you a description here but the site won’t allow us. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. #iD5-STD). U-bottom: 30-40 µl. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Figure 3. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. 0 . Features of Clear 96-Well Microplates: • Clear plates for colorimetric ELISA and other assays. The luminescence was measured in a SpectraMax. Wide range of applications The VersaMax reader covers a wide range of applications: Step 1: Turn on your microplate reader. Inconsistent results: cannot determine whole well confluence – well after well. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. Figure 2. Figure 2-23: Bottle holder To install the bottle holder: 1. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits. $3,450. . x. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Part Number. Or scan up to 384 wells at any time to determine the best assay conditions. Published Dec 16, 2020 | Updated Jun 22, 2023. Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. Description. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. 96-well-plate-scan-time-2-colors-1-site-well. Compare this item. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Part of a plate can be selected so that only a selected area of the plate. 45 µm PVDF. No accessories are needed and SoftMax® Pro Software. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. * Note: Programming the washer with a final aspirate height of 4. minutes and begin reading microplates with seven mouse clicks. #3603) SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. Fluorescence Microplate Reader Comparison Chart. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. 5% at 1. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13The microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Measurements for every well are automatically tracked over time. 20 attomol ATP per well. 2. Back plate and…. Your applications, your modes, your choice. Usage/Application: Lab. Spectrofluorometer from Molecular Devices® provides a flexible environment. These include real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR,. #3693; n = 4 for each standard). Linear, Orbital Slow, and Orbital Fast. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. We would like to show you a description here but the site won’t allow us. 0 . UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. )Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. the Molecular Devices SPECTRAmax® 190 and 250. It offers extremely low absorbance background, excellent. We would like to show you a description here but the site won’t allow us. Top or bottom detection is available with a simple click of a button. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. Microplate Capacity. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 984. May require LS-50B…. Thermomax model provides thermal control and vertical shake, perfect for. 96-, 384-, and 1536-well microplates. SpectraMax i3x Multi-Mode Microplate Reader. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. Dark blue indicates well will be read. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Top or bottom detection is available with a simple click of a button. Therefore you need to ask a physicist. 00 Add to cart; Molecular Devices EMax Microplate Reader $. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. 314 low lamp level warning. Used. for molecular biology. Selects either 96-well or 384-well plate for a stand-alone read. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 2b,c. White plates reflect the light, enabling even low levels of Luminescence to be detected. Order Now. to each well. 76358-624. They can be read by most microplate readers. With theMolecular DevicesWe designed and fabricated different sized microfluidic covers, matched to the desired experimental conditions: Fig. wells across each plate • Enables viewing and tracking of every well in every plate • Reveals additional information on cellular. Three kits covering a wide range of dsDNA concentrations. Bay Gray powder coated metal back plate, column, and base. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. Make sure toPlate reader instructions Computer. S. ©2022 Molecular Devices - All rights. EMax. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 340 PC384. T = I/I 0 and %T = 100 (T). Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. Visit the supplier page or submit a quote to learn more. of 3 wells of a 96-well plate, from 1 of 3 independent experiments. MultiWash+ for 96-well plates: 4 liquid lines, 8-way manifold and tubing, 4 wash/rinse bottles (2L), 1 waste bottle (2. Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. 00. Integrated stacker option. 2. 039. F3/F5 Multi-Mode Microplate ReadersAnalyst® Multimode Reader—one of the following: • Acquest or Analyst AD or HT with at least v. The drawing shows a standard microplate of 15 mm height covered with a lid. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. 384-WELL SBS STERILE MICROPLATE. x. 5 second read per plate. 5 OD at 492 nm Computer interface USB 2. Cross-talk <0. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. . SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. MCF7 cells treated with. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Capabilities. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. 0% ± 0. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Deep well blocks available in 24, 96 and 384-well formats An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Published Dec 16, 2020 | Updated Dec 16, 2020. The system can perform readings. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution forThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Published Dec 16, 2020 | Updated Dec 16, 2020. This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. (Molecular Devices cat. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. 1 x plates; Instrument dimensions. 190. The VersaMax reads 96-well microplates. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. #C1500) Control standard endotoxin (ACC cat. to each well. Compare this item. Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. Supplier: Molecular Devices. #E010) Pyroplate 96-well microplate (ACC cat. Access this by going to. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Working in. Absorbance cuvette port. Microplate optimization and read height optimization were performed for best results. 3% in white 96- and 384. Two high-energy xenon flash lamps: Reading capabilities. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. A = 2 - log 10 (%T). The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 96-, 384-, and 1536-well microplates. 46Click the Read button in the SoftMax Pro 8-channel (96) for FlexStation 3 Reader • 8-channel pipettor • (10) racks of 96-well, FlexStation Pipet Tips (Black) • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettor • (10) racks of 384-well, FLIPR® Tetra Pipet Tips (Clear) • 384-well yellow plate 0200-6183 Consumables ELISA Plate Readers / Microplate Readers. Including injection time, it takes approximately 68 secs. Specifications subject to change without notice. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. x. $488. ₹ 1,80,000 Get Latest Price. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Two days before the experiments, cells are seeded into the clear-bottom 96-well plates (approximately 10,000 cells per well for cancer cell lines and 30,000–40,000 cells per well for fibroblasts and NPC; see Note 5) and cultured in the media suited for users’ cell lines. It also integrates with the powerful SoftMax Pro Software for. 00. $3,207. QC tools and IQOQ services. Microplates, cuvettes (via adapter) Detectors. Can read 96 well plates (optional 384 well). 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hourMicroplate Reader, For Lab. Molecular Devices (UK) Ltd. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Molecular Devices' microplate readers. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 5 ng per well. 01. x. M2. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. For the kinetic and endpoint LAL assays, the ELx808 TM Reader is highly reliable and easy to use. External Dimensions without lid (mm).